Journal: Acta physiologica (Oxford, England)

Article Title: Early growth response 1 exacerbates thoracic aortic aneurysm and dissection of mice by inducing the phenotypic switching of vascular smooth muscle cell through the activation of Krüppel-like factor 5.

doi: 10.1111/apha.14237

Figure Lengend Snippet: FIGURE 4 Egr1 knockdown inhibited the PDGF-BB-induced phenotypic switching and migration of VSMCs. (A) The relative mRNA expression level of Egr1 normalized to β-actin in A7R5 cells transfected with pLenti-shEgr1 or pLenti-shRNA (n = 3). (B) Western blot images for protein expression of Egr1, α-SMA, SM22α, OPN, MMP2, and MMP9 in per cell group. (C) Protein expression of Egr1, α-SMA, SM22α, OPN, MMP2, and MMP9 relative to that of β-actin (n = 3). (D) Wound-healing assays were performed to assess the migratory capacity of A7R5s in each group (n = 3) (scale bar = 200 μm). (E) Quantification of the rate of migration in each group via wound-healing assay (n = 3). All data are presented as the mean ± SEM.

Article Snippet: Cells in the PDGF- BB group were cultured with 20 ng/mL PDGF- BB (HY- P7278, MedChemExpress, New Jersey, USA) for 24 h,39,40 whereas those in the control group were treated with PBS.

Techniques: Knockdown, Migration, Expressing, Transfection, shRNA, Western Blot, Wound Healing Assay

Journal: Acta physiologica (Oxford, England)

Article Title: Early growth response 1 exacerbates thoracic aortic aneurysm and dissection of mice by inducing the phenotypic switching of vascular smooth muscle cell through the activation of Krüppel-like factor 5.

doi: 10.1111/apha.14237

Figure Lengend Snippet: FIGURE 5 Egr1 knockdown inhibited the PDGF-BB-induced migration and proliferation of VSMCs. (A) Transwell assays were performed to assess the migratory capacity of A7R5s in each group (n = 3) (scale bar = 100 μm). (B) Quantification of the number of migrated cells in each group via transwell assay (n = 3). (C) Representative images of phalloidin staining for labeling F-actin in cells from each group (scale bar = 50 μm). (D) Analysis of the F-actin area and cell surface area in each group (n = 3). (E) Representative images of ki-67 (red) immunofluorescence staining. Nuclei were stained with DAPI (blue) (scale bar = 100 μm). (F) Quantification of ki-67-positive cells (n = 3). (G) Cell proliferation was detected via cell counting kit-8 (CCK-8) assay at 24, 48, and 72 h after treatment (n = 3). All data are presented as the mean ± SEM.

Article Snippet: Cells in the PDGF- BB group were cultured with 20 ng/mL PDGF- BB (HY- P7278, MedChemExpress, New Jersey, USA) for 24 h,39,40 whereas those in the control group were treated with PBS.

Techniques: Knockdown, Migration, Transwell Assay, Staining, Labeling, Immunofluorescence, Cell Counting, CCK-8 Assay

Journal: Bone

Article Title: Preservation of Type H Vessels and Osteoblasts by Enhanced Preosteoclast Platelet-Derived Growth Factor type BB Attenuates Glucocorticoid-Induced Osteoporosis in Growing Mice

doi: 10.1016/j.bone.2018.05.025

Figure Lengend Snippet: (A-B) Representative images of immunofluorescence staining of platelet-derived growth factor type BB (PDGF-BB) (green) and tartrate-resistant acid phosphatase (TRAP) (red) with quantification of number of PDGF-BB+ TRAP+ cells (yellow) in femoral primary (A) and secondary spongiosa (B) of 6 week old Ctsk+/+ wild type (WT) and Ctsk−/− mice injected with either vehicle (veh) or prednisolone 10 mg/m2/day (pred) for 4 weeks. DAPI stains nuclei blue. Scale bar, 50 μm. (C) Bone marrow PDGF-BB concentration as analyzed by ELISA. n = 6 per group. Data shown as mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: We performed PDGF-BB ELISA analysis of bone marrow supernatant using a Mouse/Rat PDGF-BB Quantikine ELISA kit (R&D Systems) according to the manufacturers’ instructions.

Techniques: Immunofluorescence, Staining, Derivative Assay, Injection, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Neoplasia (New York, N.Y.)

Article Title: Dual CTLA-4 and PD-L1 Blockade Inhibits Tumor Growth and Liver Metastasis in a Highly Aggressive Orthotopic Mouse Model of Colon Cancer 1 2

doi: 10.1016/j.neo.2019.07.006

Figure Lengend Snippet: Dual CTLA-4 and PD-L1 blockade increases the expression of Th1/M1 associated cytokines, decreases the expression of Th2/M2 related cytokines, chemokines and enzymes and increases stromal factors. Analysis of the expression of Th1/M1 ( A ), M2/Th2 ( B ) and stroma (C) associated proteins in tumors of the different treatments groups at day 14 p.i. by ELISA. Data are presented as mean ± standard deviation (* P < .05, ** P < .01, *** P < .001).

Article Snippet: IL-1α, IL-12, TGF-β, PDGF-BB, VEGF and COX-2 were measured with the Mouse IL-1 alpha/IL-1F1 DuoSet ELISA, Mouse IL-12 p70 DuoSet ELISA, Mouse TGF-β1 DuoSet ELISA, Mouse/Rat PDGF-BB DuoSet ELISA, Mouse VEGF DuoSet ELISA and Human/Mouse Total COX-2 DuoSet IC ELISA, respectively (all from R&D Systems).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Standard Deviation